EasyStep Mouse E2 (Estradiol) ELISA Kit
CAT:
855-ELK021ES-02
Size:
96 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
EasyStep Mouse E2 (Estradiol) ELISA Kit
- Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse E2 antigen, and the Mouse E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
- CAS Number: 7732-18-5
- Gene Aliases: 17B-Estradiol; Oestradiol; Beta-Estradiol
- Field of Research: Endocrinology; Reproductive science; Hormone metabolism.
- Assay Principle: Competitive Inhibition ELISA
- Assay Performance Time: 1.5h
- Standard: 1600 pg/mL
- Sample Type: Serum
- Detection Range: 25-1600 pg/mL
- Sensitivity: 25 pg/mL