EasyStep Mouse NPT (Neopterin) ELISA Kit

• Product Name Alternative:

  Neopterin

• Reactivity:

  Mouse

• Field of Research:

  Cytokine, Infection immunity

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NPT antigen, and the Mouse NPT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Mouse NPT to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse NPT in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  1.5 H

• Standard:

  30 nmol/L

• Sample Type:

  Serum, plasma

• Detection Range:

  0.469 - 30 nmol/L

• Sensitivity:

  0.4 nmol/L

• Species:

  Mouse