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EasyStep Mouse T4 (Thyroxine) ELISA Kit

CAT: 0855-ELK055ES-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK055ES-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse T4 antigen, and the Mouse T4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse T4 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Reactivity
Mouse
Field of Research
Endocrinology; Hormone metabolism
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse T4 antigen, and the Mouse T4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Mouse T4 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
1.5h
Standard
600 ng/mL
Sample Type
Serum, plasma
Detection Range
9.38-600 ng/mL
Sensitivity
7 ng/mL

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