EasyStep Mouse PG (Progesterone) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PG antigen, and the Mouse PG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse PG to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse PG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  P4; Pregn-4-Ene-3,20-Dione

• Gene Aliases:

  P4; Pregn-4-Ene-3,20-Dione

• Reactivity:

  Mouse

• Field of Research:

  Endocrinology, Reproductive science, Hormone metabolism

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PG antigen, and the Mouse PG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Mouse PG to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse PG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  1.5 H

• Standard:

  100 ng/mL

• Sample Type:

  Serum, plasma

• Detection Range:

  1.56 - 100 ng/mL

• Sensitivity:

  0.7 ng/mL

• Species:

  Mouse

• CAS Number:

  7732-18-5