EasyStep Mouse FE (Ferritin) ELISA Kit

EasyStep Mouse FE (Ferritin) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FE, and the Mouse FE standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse FE. After TMB substrate solution is added, only those wells that contain Mouse FE and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FE in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Ferritin
• Field of Research: Metabolic pathway; Tumor immunity; Hematology.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 1.5h
• Standard: 40 ng/mL
• Sample Type: Serum
• Detection Range: 0.63-40 ng/mL
• Sensitivity: 6.4 pg/mL