Anti-Lhca2 | PSI type II chloropyll a/b-binding protein

Anti-Lhca2 | PSI type II chloropyll a/b-binding protein

• Background: The light-harvesting proteinLhca2is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca2 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca2 forms a heterodimer (LHCI-680) with Lhca3 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found inKlimmeket al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Arachis hypogaea, Bryopsis corticulans,Colobanthus quitensisKunt Bartl, Chlamydomonas reinhardti (one Lhca-type), Citrus reticulata, Chromochloris zofingiensis, Cytisus cantabricus(Wilk.) Rchb. F.,Hieracium pilosellaL,Hordeum vulgare, Lasallia hispanica, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Physcomitrium patens, Pinus banksiana(the higher of the two bands detected at 24 and 30 kDa is not considered to be specific to any Lhc protein),Posidonia oceanica,Prasinodermasp.,Pyramimonassp.Spinacia oleracea, Syntrichia muralis(Hedw.) Raab, Triticum aestivum, Triticale, Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: BSA-conjugated synthetic peptide derived from the Lhca2 protein ofArabidopsis thalianaUniProt:Q9SYW8,Q8LCQ4, TAIR:At3g61470. This sequence is highly conserved in Lhca2 proteins of angiosperms (monocots and dicots) and gymnosperms as well as inAt1g19150. This gene codes for the very low expressed Lhca6 protein which also has been denoted as Lhca2*1.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 2000-1 : 5000 (WB)
• Purity: Total IgG. Protein G purified in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 100 µl of sterile water
• Molecular Weight: 27.7 | 24 kDa forArabidopsis thaliana
• References & Citations: Zhaoet al. (2024). Psb28 protein is indispensable for stable accumulation of PSII core complexes in Arabidopsis.Plant J. 2024 May 26. doi: 10.1111/tpj.16844.Sarvariet al. (2022). Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE. Plant Methods. 2022 Mar 3;18(1):23. doi: 10.1186/s13007-022-00858-2. PMID: 35241118; PMCID: PMC8895881.Fukuraet al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Zhuet al. (2020). A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata. J Exp Bot. 2020 Jun 22;71(12):3613-3625.doi: 10.1093/jxb/eraa118.Theiret al. (2020). VIPP2 interacts with VIPP1 and HSP22E/F at chloroplast membranes and modulates a retrograde signal for HSP22E/F gene expression. Plant Cell Environ. 2020 Jan 29. doi: 10.1111/pce.13732.Vojtaand Fulgosi (2019). Topology of TROL protein in thylakoid membranes of Arabidopsis thaliana. Physiol Plant. 2019 Jan 20. doi: 10.1111/ppl.12927.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.