Anti-Lhcb3 | LHCII type III chlorophyll a/b-binding protein

Anti-Lhcb3 | LHCII type III chlorophyll a/b-binding protein

• Background: The major light-harvesting antenna complex II (LHCII)in photsynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. While Lhcb1 and Lhcb2 are quite similar and regularily present in multiple gene-copies, theLhcb3protein differs in pigment-composition and molecular size and often is coded by only a single gene. Lhcb3 seems not to be present in the mobile LHCII trimers involved in state 1-state 2 transitions.A molecular characterisation of the LHCII proteins can be found inCaffarriet al. (2004) A Look within LHCII:  Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Arachis hypogaea, Chlorella vulgaris, Cucumis sativa, Dactylis glomeRata, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Physcomirella patens, Prasinoderma sp.,Pyramimonassp., Spinacia oleracea, Triticum aestivum, Triticale, Zea may, Verbascum lychnitis
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms, includingArabidopsis thalianaLhcb3 UniProt:Q9S7M0,TAIR:AT5G54270. This sequence is highly conserved even inGinko bilobaand one of the major LHCII-forms ofPhyscomitrella patens.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 2000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 28.7 | 26 kDa forArabidopsis thaliana
• Precautions: Protein is processed into mature form (Jansson1999).
• References & Citations: Ciesielskaet al. (2024). S2P2-the chloroplast-located intramembrane protease and its impact on the stoichiometry and functioning of the photosynthetic apparatus of A. thaliana. Front Plant Sci. 2024 Mar 15:15:1372318. doi: 10.3389/fpls.2024.1372318.Wuet al (2023) Disruption of LEAF LESION MIMIC 4 affects ABA synthesis and ROS accumulation in rice.Leisteret al (2023) An ancient metabolite damage-repair system sustains photosynthesis in plants.vonBismarck, et al (2023). Light acclimation interacts with thylakoid ion transport to govern the dynamics of photosynthesis in Arabidopsis. New Phytol. 2023;237(1):160-176. doi:10.1111/nph.18534Liet al. (2022) The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants. Cell Rep. 2022 Apr 12;39(2):110664. doi: 10.1016/j.celrep.2022.110664. PMID: 35417702.Bru, Steen, Park, et al. (2022) The major trimeric antenna complexes serve as a site for qH-energy dissipation in plants. J Biol Chem. 2022;298(11):102519. doi:10.1016/j.jbc.2022.102522Ivanovet al. (2022) The decreased PG content of pgp1 inhibits PSI photochemistry and limits reaction center and light-harvesting polypeptide accumulation in response to cold acclimation. Planta 255, 36 (2022). https://doi.org/10.1007/s00425-022-03819-0vonBismarck et al. (2021) Light acclimation interacts with thylakoid ion transport to govern the dynamics of photosynthesis. Research Square; 2021. DOI: 10.21203/rs.3.rs-948381/v1.Wuet al. (2021). Formation of light-harvesting complex (LHC) II aggregates from LHCII-PSI-LHCI complexes in rice plants under high light. J Exp Bot. 2021 May 3:erab188. doi: 10.1093/jxb/erab188. Epub ahead of print. PMID: 33939808.Wojtowiczet al. (2020). Compensation Mechanism of the Photosynthetic Apparatus in Arabidopsis thaliana ch1 Mutants. Int J Mol Sci. 2020 Dec 28;22(1):221. doi: 10.3390/ijms22010221. PMID: 33379339; PMCID: PMC7794896.Kohet al. (2019). Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency. Biotechnol Biofuels. 2019 May 14;12:122. doi: 10.1186/s13068-019-1462-3. eCollection 2019.Furukawaet al. (2019). Formation of a PSI–PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens. J Plant Reshttps://doi.org/10.1007/s10265-019-01138-2.Lvet al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Rogowskiet al. (2019). Photosynthesis and organization of maize mesophyll and bundle sheath thylakoids of plants grown in various light intensities. Environmental and Experimental Botany Volume 162, June 2019, Pages 72-86.Maoet al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.Rantalaand Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Myougaet al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.Shinet al. (2017), Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci Rep. 2017 Dec 20;7(1):17929. doi:10.1038/s41598-017-18221-0.Tyuerevaet al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)Yang-ErChen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.Rozpadeket al. (2015). The fungal endophyte Epichloë typhina improves photosynthesis efficiency of its host orchard grass (Dactylis glomerata). Planta. 2015 Jun 10.Yokonoet al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.Yaoet al. (2015). Ultraviolet-B protection of ascorbate and tocopherol in plants related with their function on the stability on carotenoid and phenylpropanoid compounds. Plant Physiology and Biochemistry Volume 90, May 2015, Pages 23–31.Kunugiet al. (2016). Evolution of Green Plants Accompanied Changes in Light-Harvesting Systems. Plant Cell Physiol. 2016 Apr 6. pii: pcw071. Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.Wientjeset al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.Rudowskaet al. (2012). Chloroplast biogenesis - correlation between structure and function. BBA, available on line, March 2012.Lanet al (2023) UPL5 modulates WHY2 protein distribution in a Kub-site dependent ubiquitination in response to [Ca2+]cyt-induced leaf senescence
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.