Anti-Lhcb1 | LHCII type I chlorophyll a/b-binding protein
CAT:
451-AS01 004
Size:
50 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No

Anti-Lhcb1 | LHCII type I chlorophyll a/b-binding protein
- Background: The major light-harvesting antenna complex II (LHCII)in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3.Lhcb1is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.A molecular characterisation of the LHCII proteins can be found inCaffarriet al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476
- CAS Number: 9007-83-4
- Host: Rabbit
- Reactivity: Arabidopsis thaliana, Arachis hypogaea, Brassica napus, Camelina sinensis, Citrus retuculata, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Rhoeo discolor, Silene vulgaris, Sinapsis alba, Solanum lycopersicum, Spinacia oleracea, Tillandsia flabellate, Triticum aestivum, Triticale, Zea mays
- Not reactive in: Picea abies
- Immunogen: BSA-conjugated, 11 amino acidslong synthetic peptide derived from a highly conserved sequence of Lhb1 proteins from angiosperms (monocots and dicots) and gymnosperms, includingArabidopsis thalianaLhcb1.1 UniProt:P0CJ48-1, TAIR:AT1G29920, Lhcb1.2 UniProt:Q8VZ87-1, TAIR:AT1G29910, Lhcb1.3 (most expressed) UniProt:P04778-1, TAIR:AT1G2993, Lhcb1.4 UniProt:Q39142-1, TAIR:AT2G34430, Lhcb1.5 UniProt:Q39141-1, TAIR:At2g34420
- Clonality: Polyclonal
- Applications: Western blot (WB)
- Dilution: 1 : 2000 (WB)
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Reconstitution: For reconstitution add 50 µl of sterile water
- Molecular Weight: 28 | 25 kDa forArabidopsis thaliana
- Precautions: Lhcb1 Protein is processed into mature form (Jansson1999).This product can be sold containing ProClin if requested
- References & Citations: Sulliet al. (2023). Generation and physiological characterization of genome‑edited Nicotiana benthamiana plants containing zeaxanthin as the only leaf xanthophyll. Planta . 2023 Oct 5;258(5):93. doi: 10.1007/s00425-023-04248-3.Yeet al. (2023). The light-harvesting chlorophyll a/b-binding proteins of photosystem II family members are responsible for temperature sensitivity and leaf color phenotype in albino tea plant. J Adv Res . 2023 Dec 25:S2090-1232(23)00404-6.doi: 10.1016/j.jare.2023.12.017.Huet al. (2023). Drought affects both photosystems in Arabidopsis thaliana. New Phytol. 2023 Oct;240(2):663-675. doi: 10.1111/nph.19171. Epub 2023 Aug 2.HuC, Mascoli V, Elias E, Croce R. The photosynthetic apparatus of the CAM plant Tillandsia flabellate and its response to water deficit. J Plant Physiol. 2023 Mar;282:153945. doi: 10.1016/j.jplph.2023.153945Jinet al. (2023) Dual roles for CND1 in maintenance of nuclear and chloroplast genome stability in plants. Cell Rep. 2023 Mar 28;42(3):112268. doi: 10.1016/j.celrep.2023.112268. Epub 2023 Mar 17.BoussardonC, Carrie C, Keech O. Comparing plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells. J Exp Bot. 2023 Apr 7:erad133. doi: 10.1093/jxb/erad133.Harchouniet al. (2022) Guanosine tetraphosphate (ppGpp) accumulation inhibits chloroplast gene expression and promotes super grana formation in the moss Physcomitrium (Physcomitrella) patens. New Phytol. 2022;236(1):86-98. doi:10.1111/nph.18320GaoY et al. (2022) Chloroplast translational regulation uncovers nonessential photosynthesis genes as key players in plant cold acclimation. Plant Cell. 2022 Apr 26;34(5):2056-2079. doi: 10.1093/plcell/koac056. PMID: 35171295; PMCID: PMC9048916.Ivanovet al. (2022) The decreased PG content of pgp1 inhibits PSI photochemistry and limits reaction center and light-harvesting polypeptide accumulation in response to cold acclimation. Planta 255, 36 (2022). https://doi.org/10.1007/s00425-022-03819-0Medina-Pucheet al (2021). Protocol for evaluating protein relocalization from the plasma membrane to chloroplasts. STAR Protoc. 2021 Sep 14;2(4):100816. doi: 10.1016/j.xpro.2021.100816. PMID: 34585156; PMCID: PMC8450296.Chen, Liu & Liu (2021) Loss-Function of EGY1 Results in Photosynthesis Damage through Reducing Stability of Photosystem II in Arabidopsis thaliana. Russ J Plant Physiol (2021).Wuet al. (2021). Formation of light-harvesting complex (LHC) II aggregates from LHCII-PSI-LHCI complexes in rice plants under high light. J Exp Bot. 2021 May 3:erab188. doi: 10.1093/jxb/erab188. Epub ahead of print. PMID: 33939808.Loudyaet al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.Shuklaet al. (2020). A novel method produces native LHCII aggregates from the photosynthetic membrane revealing their role in non-photochemical quenching. J Biol Chem. 2020 Oct 20:jbc.RA120.016181. doi: 10.1074/jbc.RA120.016181. Epub ahead of print. PMID: 33082138.Zhuet al. (2020). A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata. J Exp Bot. 2020 Jun 22;71(12):3613-3625.doi: 10.1093/jxb/eraa118.Forlaniet al. (2020. HEBE, a novel positive regulator of senescence in Solanum lycopersicum. Sci Rep. 2020 Jul 3;10(1):11021.doi: 10.1038/s41598-020-67937-z.Wanget al. (2020). Effects and Mechanisms of Foliar Application of Silicon and Selenium Composite Sols on Diminishing Cadmium and Lead Translocation and Affiliated Physiological and Biochemical Responses in Hybrid Rice (Oryza Sativa L.) Exposed to Cadmium and Lead. Chemosphere. 2020 Jul;251:126347. doi: 10.1016/j.chemosphere.2020.126347.Galviset al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.Averinaet al. (2019). Photosynthesis and Oxygen Uptake Rate in Winter Rape Plants Treated with 5-Aminolevulinic Acid. Russian Journal of Plant Physiology volume 66, pages966–975(2019).Kohet al. (2019). Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency. Biotechnol Biofuels. 2019 May 14;12:122. doi: 10.1186/s13068-019-1462-3. eCollection 2019.Rogowskiet al. (2019). Photosynthesis and organization of maize mesophyll and bundle sheath thylakoids of plants grown in various light intensities. Environmental and Experimental Botany Volume 162, June 2019, Pages 72-86.Lvet al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Chenet al. (2018). TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018 Dec;564(7734):125-129. doi: 10.1038/s41586-018-0713-y.Maoet al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.Giovanardiet al. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta. 2018 May 24. pii: S0005-2728(18)30129-4. doi: 10.1016/j.bbabio.2018.05.013.Myougaet al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.Rantalaet al. (2017). Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane. Plant J. 2017 Dec;92(5):951-962. doi: 10.1111/tpj.13732.Shinet al. (2017), Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci Rep. 2017 Dec 20;7(1):17929. doi: 10.1038/s41598-017-18221-0.Yang-ErChen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.Mazuret al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4.Kowalewskaet al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015.Fristedtet al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.Armbrusteret al. (2014). Ion antiport accelerates photosynthetic acclimation in fluctuating light environments. Nat Commun. 2014 Nov 13;5:5439. doi: 10.1038/ncomms6439
- Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.