Anti-Deg1 | chloroplastic DegP-type serine protease 1

• Background:

  Deg1belongs to S1 chymotrypsin protease family and contains 16 protein members inArabidopsis thaliana. Deg1 is localized to chloroplast.

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Pisum sativum, Zea mays

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Recombinant Deg1 ofArabidopsis thalianaO22609,At3g27925, overexpressed inE.coli

• Clonality:

  Polyclonal

• Applications:

  Western blot (WB)

• Dilution:

  1 : 500/5 ug thylakoid fraction (WB)

• Purity:

  Total IgG. Protein G purified in PBS pH 7.4.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 200 µl of sterile water

• Molecular Weight:

  46.6 | 36 kDa

• Precautions:

  Unspecific reaction at ca. 30 kDa and ca. 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease, The band at the right MW does contain Deg1 by our MS analysis

• References & Citations:

  Zienkiewiczet al. (2013). Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plant Physiol. & Biochem. March 16.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4