Anti-NdbA | Thylakoid Localized Type 2 NAD(P)H Dehydrogenase

Anti-NdbA | Thylakoid Localized Type 2 NAD(P)H Dehydrogenase

• Background: NdbA (Thylakoid Localized Type 2 NAD(P)H Dehydrogenase)is localized to the thylakoid membrane and expressed at a very low level in the widl type under photoautotrophic growth. NdbA is induced substantially under light-activated heterotophic growth conditions.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Synechocystissp. PCC 68
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated peptide derived from NdbA protein sequence ofSynechocystissp. PCC 6803, UniProt:P73739Chosen peptide is not conserved in NdbB and NdbC.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 2000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl, of sterile water
• Molecular Weight: 49 | 46 kDa
• Precautions: Protein extraction: harvested cells were suspended in a buffer containing 50 mM Hepes-NaOH, pH 7,5, 30 mM CaCl2, 800 mM sorbitol, 1 mM ε-amino-n-caproic acid, and the cells were broken by vortexing 6×1 min at 4°C in the presence of glass beads, Protein samples were solubilized in Laemmli buffer containing 6 M urea and separated on a gel in presence of 6M urea
• References & Citations: Huokkoet al. (2019). Thylakoid Localized Type 2 NAD(P)H Dehydrogenase NdbA Optimizes Light-Activated Heterotrophic Growth of Synechocystis sp. PCC 6803. Plant Cell Physiol. 2019 Mar 7. pii: pcz044. doi: 10.1093/pcp/pcz044.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.