Anti-Lhca3 | PSI type III chlorophyll a/b-binding protein

Anti-Lhca3 | PSI type III chlorophyll a/b-binding protein

• Background: The light-harvesting proteinLhca3is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca3 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca3 forms a heterodimer (LHCI-680) with Lhca2 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found inKlimmeket al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Aracis hypogae, Bryopsis corticulans, Citrus reticulata, Hordeum vulgare, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Posidonia oceanica, Spinacia oleracea, Triticum aestivum, Triticale, Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence fromArabidopsis thalianaUniProt:Q9SY97, TAIR:At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 2000-1 : 5000 (WB)
• Purity: Total IgG. Protein G purified in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 100 µl of sterile water
• Molecular Weight: 29 | 25 kDa forArabidopsis thaliana(due to a transit peptide being cleaved off)
• Precautions: Protein is processed into mature form (Jansson1999).
• References & Citations: Sarvariet al. (2022). Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE. Plant Methods. 2022 Mar 3;18(1):23. doi: 10.1186/s13007-022-00858-2. PMID: 35241118; PMCID: PMC8895881.Zhuet al. (2020). A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata. J Exp Bot. 2020 Jun 22;71(12):3613-3625.doi: 10.1093/jxb/eraa118.Chenet al. (2019). Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat. Int J Mol Sci. 2019 Dec 31;21(1). pii: E268. doi: 10.3390/ijms21010268.Maoet al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.Liet al. (2018). Modulating plant growth-metabolism coordination for sustainable agriculture. Nature. 2018 Aug 15. doi: 10.1038/s41586-018-0415-5.Myougaet al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.Schottleret al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Chenet al. (2017). Comparison of Photosynthetic Characteristics and Antioxidant Systems in Different Wheat Strains. J Plant Growth Regul.Tyuerevaet al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot).Yokonoet al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.Qinet al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.