Anti-Lhcb2-P | LHCII type II chlorophyll a/b-binding protein, phosphorylated

Anti-Lhcb2-P | LHCII type II chlorophyll a/b-binding protein, phosphorylated

• Background: The major light-harvesting antenna complex II (LHCII)in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3.Lhcb2is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Asterochloris erici (lichen photobiont), Echinochloa crus-galli,Oryza sativa, , Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide: RRT*VKSTPQS, where T* indicates phospho-Thr
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 10 000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 25 µl of sterile water
• Molecular Weight: 25 | 25 kDa forArabidopsis thaliana
• References & Citations: Virtanen, Tyystjarvi, (2023). Plastoquinone pool redox state and control of state transitions in Chlamydomonas reinhardtii in darkness and under illumination. Photosynth Res. 2023;155(1):59-76. doi:10.1007/s11120-022-00970-3.Rantalaet al. (2022) Chloroplast Acetyltransferase GNAT2 is Involved in the Organization and Dynamics of Thylakoid Structure. Plant Cell Physiol. 2022 Sep 15;63(9):1205-1214. doi: 10.1093/pcp/pcac096. PMID: 35792507; PMCID: PMC9474947.Wuet al. (2021). Formation of light-harvesting complex (LHC) II aggregates from LHCII-PSI-LHCI complexes in rice plants under high light. J Exp Bot. 2021 May 3:erab188. doi: 10.1093/jxb/erab188. Epub ahead of print. PMID: 33939808.Mazuret al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.Bychkovet al. (2019). Melatonin modifies the expression of the genes for nuclear- and plastid-encoded chloroplast proteins in detached Arabidopsis leaves exposed to photooxidative stress. Plant Physiology and Biochemistry, doi.org/10.1016/j.plaphy.2019.10.013.Vietoshkinaet al. (2019). Comparison of State Transitions of the Photosynthetic Antennae in Arabidopsis and Barley Plants upon Illumination with Light of Various Intensity. Biochemistry (Moscow), Vol 84, Issue 9, pp 1065–1073Rudenkoet al. (2019). The role of carbonic anhydrase ?-CA4 in the adaptive reactions of photosynthetic apparatus: the study with ?-CA4 knockout plants. Protoplasma (2019).https://doi.org/10.1007/s00709-019-01456-1.Gasullaet al. (2018). Chlororespiration induces non-photochemical quenching of chlorophyll fluorescence during darkness in lichen chlorobionts. Physiol Plant. 2018 Jun 27. doi: 10.1111/ppl.12792.Rantalaand Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Rantalaet al. (2017). Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane. Plant J. 2017 Dec;92(5):951-962. doi: 10.1111/tpj.13732.Fristedtet al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218.Satoet al. (2015). Chlorophyll b degradation by chlorophyll b reductase under high-light conditions. Photosynth Res. 2015 Apr 21.Leoniet al. (2013). Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis. Plant J. Oct;76(2):236-46. doi: 10.1111/tpj.12297. Epub 2013 Aug 26.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.