Anti-Lhcb1-P | LHCII type I chlorophyll a/b-binding protein, phopshorylated

Anti-Lhcb1-P | LHCII type I chlorophyll a/b-binding protein, phopshorylated

• Background: The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3.Lhcb1is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Echinochloa crus-galli,, Oryza sativa, Panicum miliaceum, Pisum sativum, Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide RKT*VAKPKGP, where T* indicates phospho-Thr
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 10 000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 25 µl of sterile water
• Molecular Weight: 25 | 25 kDa forArabidopsis thaliana
• References & Citations: Rantalaet al. (2022) Chloroplast Acetyltransferase GNAT2 is Involved in the Organization and Dynamics of Thylakoid Structure. Plant Cell Physiol. 2022 Sep 15;63(9):1205-1214. doi: 10.1093/pcp/pcac096. PMID: 35792507; PMCID: PMC9474947.Nilssonet al. (2020). PSB33 protein sustains Photosystem II in plant chloroplasts under UVA light. J Exp Bot. 2020 Sep 15;eraa427.doi: 10.1093/jxb/eraa427.Rudenkoet al. (2019). The role of carbonic anhydrase a-CA4 in the adaptive reactions of photosynthetic apparatus: the study with a-CA4 knockout plants. Protoplasma (2019). https://doi.org/10.1007/s00709-019-01456-1Rantalaand Tikkanen et al. (2018). Phosphorylation-induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Rantalaet al. (2017). Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane. Plant J. 2017 Dec;92(5):951-962. doi: 10.1111/tpj.13732.Fristedtet al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218.Schonberget al. (2017). Identification of STN7/STN8 kinase targets reveals connections between electron transport, metabolism and gene expression. Plant J. 2017 Jun;90(6):1176-1186. doi: 10.1111/tpj.13536.Longoniet al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015.Satoet al. (2015). Chlorophyll b degradation by chlorophyll b reductase under high-light conditions. Photosynth Res. 2015 Apr 21.Jiaet al. (2014). Accumulation of NON-YELLOW COLORING 1 protein of the chlorophyll cycle requires chlorophyll b in Arabidopsis thaliana. Plant J. 2014 Dec 30. doi: 10.1111/tpj.12753.Leoniet al. (2013). Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis. Plant J. Oct;76(2):236-46. doi: 10.1111/tpj.12297. Epub 2013 Aug 26.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.