Recombinant E.coli Tryptophan Synthase β Chain/Trp B Protein (His Tag)

Recombinant E.coli Tryptophan Synthase β Chain/Trp B Protein (His Tag)

• Background: Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.
• Abbreviation: Tryptophan Synthase β Chain;Trp B
• Synonyms: Tryptophan synthase beta chain, trpB
• CAS Number: 9000-83-3
• UniProt: P0A879
• Accession Number: P0A879
• Host: E. coli
• Origin Species: E.coli
• Tag: N-His
• Sequence: Thr2-Ile397
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: Not Applicable
• Molecular Weight: 38-48 kDa
• Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
• Storage Conditions: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.