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Recombinant Human LIF Protein (E.coli)

CAT: 0763-PKSH032694-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0763-PKSH032694-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation; control of stem cell pluripotency; bone and fat metabolism; mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Abbreviation
LIF
Synonyms
CDF; D Factor; DIA; Differentiation-Stimulating Factor; Emfilermin; HILDA; LIF; Leukemia Inhibitory Factor; MLPLI; Melanoma-Derived LPL Inhibitor
UniProt
P15018
Accession Number
P15018
Expression System
E.coli
Tag
N-His
Sequence
Ser23-Phe202
Applications
Cell culture
Field of Research
Cell biology; Neuroscience; Cancer; epigenetics and nuclear signal; immunology
Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method.
Purity
> 98 % as determined by reducing SDS-PAGE.
Bioactivity
50
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
20.5 kDa
Observed Molecular Weight
18 kDa
Species
Human