Recombinant E.coli Tryptophan Synthase Protein (His Tag)

Recombinant E.coli Tryptophan Synthase Protein (His Tag)

• Background: Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3′-phosphate (IGP) to give indole and D-glyceraldehyde 3′-phosphate (G3P). Indole is then transferred through a 25-hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.
• Abbreviation: Tryptophan Synthase
• Synonyms: Tryptophan synthase, Tryptophan synthetase
• CAS Number: 9000-83-3
• UniProt: P0A877;P0A879
• Accession Number: P0A877&P0A879
• Host: E. coli
• Origin Species: E.coli
• Tag: N-His
• Sequence: Met1-Ser268&Thr2-Ile397
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: Not Applicable
• Molecular Weight: 28&40-50 kDa
• Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
• Storage Conditions: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.