Recombinant E.coli Beta-galactosidase Protein

• Background:

  β-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. β-galactosides include carbohydrates containing galactose where the glycosidic bond lies above the galactose molecule. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. It is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of β-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. β-galactosidase is important for organisms as it is a key provider in the production of energy and a source of carbons through the break down of lactose to galactose and glucose.

• Abbreviation:

  Beta-galactosidase

• Synonyms:

  Beta-gal; Beta-galactosidase; Lactase; lacZ

• UniProt:

  P00722

• Accession Number:

  P00722

• Expression System:

  E. coli

• Tag:

  None

• Sequence:

  Met1-Lys1024 (12-41AA deletion)

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  112.9 kDa

• Observed Molecular Weight:

  115 kDa

• Formulation:

  Supplied as a 0.2 µm filtered solution of PBS, pH7.4.

• Species:

  E.coli