MHC Class 1, H2, Kk (FITC)
-
Catalog number
MBS600708
-
Price
Please ask
-
Size
5x0.5 mg
-
-
Products_type
Antibody
-
Products_short_name
[MHC Class 1, H2, Kk]
-
Products_name_syn
[Anti -MHC Class 1, H2, Kk (FITC)]
-
Clonality
Monoclonal
-
Clone
[5K51]
-
Reactivity
Mouse
-
Specificity
Reacts with the H-2Kk MHC class I alloantigen. Crossreactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. Does not react with other (e.g., b, d, q) haplotypes.
-
Purity
Purified Purified
-
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
-
Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
-
Tested_application
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
-
Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
-
Conjugation
Anti-FITC Antibody
-
Gene target
-
Short name
MHC Class 1, H2, Kk (FITC)
-
Technique
FITC, Fluorescein
-
Host
Mouse
-
Isotype
IgG2a,k
-
Label
FITC
-
Alternative name
Major histocompabitility complex Class 1, H2, Kk (fluorecein)
-
Alternative technique
fluorescine
-
MeSH Data
-
Name
-
Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products