MHC Class I antibody FITC
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Catalog number
61R-1165
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Price
Please ask
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Size
50 µg
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Category
Primary Antibody
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Antibody Subtype
Conjugated Monoclonal Antibodies
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Area of research
Immunology
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Raised in
Mouse
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Specificity
Mouse
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Isotypes
IgG2a kappa
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Clone
28-14-8
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Conjugate Tag
FITC
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Concentration
500 ug/ml
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Form Buffer
Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
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Storage
Store at 2-8 deg C. Do not freeze. Protect from light.
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Shipping conditions
Blue Ice
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Tested for
FC
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Usage Recommendations
FC: ≤0.25 ug/test
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Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
MICD, MICF, MICG
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Short name
MHC Class I antibody FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Mouse monoclonal MHC Class I antibody (FITC)
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Alternative technique
antibodies, fluorescine
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Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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