MHC Class I antibody FITC
-
Catalog number
61R-1164
-
Price
Please ask
-
Size
100 µg
-
-
Category
Primary Antibody
-
Antibody Subtype
Conjugated Monoclonal Antibodies
-
Area of research
Immunology
-
Raised in
Mouse
-
Specificity
Mouse
-
Isotypes
IgG2a kappa
-
Clone
34-1-2S
-
Conjugate Tag
FITC
-
Concentration
500 ug/ml
-
Form Buffer
Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
-
Storage
Store at 2-8 deg C. Do not freeze. Protect from light.
-
Shipping conditions
Blue Ice
-
Tested for
FC
-
Usage Recommendations
FC: ≤0.25 ug/test
-
Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
-
-
-
Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
-
Conjugation
Anti-FITC Antibody
-
French translation
anticorps
-
Gene target
-
Gene symbol
MICD, MICF, MICG
-
Short name
MHC Class I antibody FITC
-
Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
-
Label
FITC
-
Alternative name
Mouse monoclonal MHC Class I antibody (FITC)
-
Alternative technique
antibodies, fluorescine
-
Gene info
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products