MHC Class I antibody FITC
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Catalog number
61R-M109aFT
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Price
Please ask
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Size
120 tests
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Category
Primary Antibody
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Antibody Subtype
Conjugated Monoclonal Antibodies
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Area of research
Immunology
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Type of Immunogen
MHC Class I antibody (FITC) was raised in mouse using human HSB-2 T cell line ..
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Raised in
Mouse
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Specificity
Human
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Cross Reactivity
Should be determined by the end user
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Isotypes
IgG2a
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Clone
3F10
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Conjugate Tag
FITC
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Method of Purification
MHC Class I antibody (FITC) was purified by Protein A chromatography.
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Concentration
0.25 mg/ml
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Form Buffer
50 mM Na3PO4, pH 7.5, with 100 mM KCL, 150 mM NaCl, 5% Glycerol, 0.2% BSA, and 0.04% NaN3.
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Storage
Store at 4 deg C. Keep away from light.
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Shipping conditions
Blue Ice
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Tested for
ELISA; IHC; FC
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Usage Recommendations
FC: 5 ug/ml
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Additional Information
This antibody recognizes a monomorphic Class I epitope on the alpha chain.
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
MICD, MICF, MICG
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Short name
MHC Class I antibody FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Mouse monoclonal MHC Class I antibody (FITC)
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Alternative technique
antibodies, fluorescine
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Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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