MHC Class II antibody FITC
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Catalog number
61R-M110aFT
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Price
Please ask
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Size
120 tests
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Category
Primary Antibody
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Antibody Subtype
Conjugated Monoclonal Antibodies
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Area of research
Immunology
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Type of Immunogen
MHC class II antibody (FITC) was raised in mouse using purified class II from JY cell line ..
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Raised in
Mouse
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Specificity
Human
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Cross Reactivity
Should be determined by the end user
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Isotypes
IgG1
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Clone
TDR31.1
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Conjugate Tag
FITC
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Method of Purification
MHC Class II beta chain antibody (FITC) was purified by Protein A chromatography.
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Concentration
0.25 mg/ml
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Form Buffer
50 mM Na3PO4, pH 7.5, with 100 mM KCL, 150 mM NaCl, 5% Glycerol, 0.2% BSA, and 0.04% NaN3.
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Storage
Store at 4 deg C. DO NOT FREEZE.
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Shipping conditions
Blue Ice
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Tested for
FC; WB
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Usage Recommendations
FC: 5 ug/ml
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Additional Information
This antibody recognizes a monomorphic class II beta chain epitope.
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
CD74, BTNL2, RFX1
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Short name
MHC Class II antibody FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Mouse monoclonal MHC Class II antibody (FITC)
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Alternative technique
antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene name
CD74 molecule
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Synonyms gene
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Synonyms gene name
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
- CD74 molecule, major histocompatibility complex, class II invariant chain
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Synonyms
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Synonyms name
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
butyrophilin like 2
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Synonyms gene name
- butyrophilin-like 2 (MHC class II associated)
- butyrophilin-like 2
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Butyrophilins
- C2-set domain containing
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
regulatory factor X1
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Synonyms gene name
- regulatory factor X, 1 (influences HLA class II expression)
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Synonyms
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Synonyms name
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Locus
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Discovery year
1991-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Regulatory factor X family
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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