MHC Class II antibody FITC
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Catalog number61R-1137
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Price:
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Size50 µg
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchImmunology
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Raised inRat
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SpecificityMouse
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IsotypesIgG2b kappa
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CloneM5/114.15.2
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Conjugate TagFITC
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Concentration500 ug/ml
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Form BufferProvided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
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StorageStore at 2-8 deg C. Do not freeze. Protect from light.
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Shipping conditionsBlue Ice
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Tested forFC
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Assay Informationug size: has been tested by flow cytometric analysis of human peripheral blood leukocytes and should be used at the same concentration as the experimental antibody.
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Additional InformationThis antibody is offered in 2 formats.
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolCD74, BTNL2, RFX1
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Short nameMHC Class II antibody FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameRat monoclonal MHC Class II antibody (FITC)
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameCD74 molecule
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Synonyms gene
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Synonyms gene name
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
- CD74 molecule, major histocompatibility complex, class II invariant chain
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Synonyms
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebutyrophilin like 2
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Synonyms gene name
- butyrophilin-like 2 (MHC class II associated)
- butyrophilin-like 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Butyrophilins
- C2-set domain containing
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameregulatory factor X1
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Synonyms gene name
- regulatory factor X, 1 (influences HLA class II expression)
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Regulatory factor X family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data