Biotin antibody
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Catalog number10-1888
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PricePlease ask
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Size200 µl
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CategoryPrimary Antibody
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Antibody SubtypeMonoclonal Antibodies
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Area of researchCell Biology
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Type of ImmunogenBiotin antibodies were raised in Mouse using Biotinylated denatured ovalbumin .
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Raised inMouse
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Specificity10-1888 is specific for biotin
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IsotypesIgG2a kappa
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CloneM81504
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Method of PurificationBiotin antibody was purified from cell culture supernatant by protein A/G chromatography
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Form BufferProvided in 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM NaN3
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StorageStore at 4-8 deg C without exposure to light
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Shipping conditionsBlue Ice
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Tested forELISA; WB
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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French translationanticorps
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Short nameantibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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LabelBiotin
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Alternative nameMouse monoclonal Biotin antibody
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data