Biotin antibody

Category

Primary Antibody
Antibody Subtype

Polyclonal Antibodies
Area of research

Miscellaneous
Type of Immunogen

Biotin antibodies were raised in goat using biotin conjugated to KLH ..
Raised in

Goat
Cross Reactivity

This serum reacts only to Biotin
Method of Purification

Biotin antibody was purified by ion exchange chromatography followed by dialysis.
Form Buffer

Monospecific antisera, absorbed, delipidated and defibrinated, Provided in 0.02M NaPO4, 0.15M NaCl, pH 7.2, with 0.01% NaN3
Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditions

Blue Ice
Tested for

ELISA; IHC; WB
Usage Recommendations

ELISA: 1:100,000-1:500,000, WB: 1:10,000-1:50,000
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data