Biotin antibody
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Catalog number70R-7551
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PricePlease ask
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Size1 mg
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CategoryPrimary Antibody
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Antibody SubtypePolyclonal Antibodies, Purified
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Area of researchMiscellaneous
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Type of ImmunogenBiotin
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Raised inGoat
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Grade PurityGrater than 95% pure
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Method of PurificationAffinity purified using solid phase biotin
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SourceGoat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration>4.5 mg/ml (lot specific)
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Form BufferClear, colorless, liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
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StorageStore at 2-8 deg C.
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Shipping conditionsBlue Ice
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Tested forPlease refer to the datasheet
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Usage RecommendationsThe optimal working dilution should be determined by the end user
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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French translationanticorps
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Short nameantibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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LabelBiotin
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Alternative nameBiotin antibody
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data