Biotin antibody rhodamine

Category

Primary Antibody
Antibody Subtype

Conjugated Polyclonal Antibodies
Area of research

Miscellaneous
Type of Immunogen

Biotin
Raised in

Goat
Conjugate Tag

Rhodamine
Grade Purity

Grater than 95% pure
Method of Purification

Affinity purified using solid phase biotin
Source

Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Concentration

1.0 mg/ml (lot specific)
Form Buffer

Lyophilized from 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2. Dilute in dH2O to a concentration of 1 mg/ml
Storage

Store at 2-8 deg C.
Shipping conditions

Blue Ice
Tested for

FC; IF; IHC
Usage Recommendations

The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data