Biotin antibody
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Catalog number20C-CR7026RP
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PricePlease ask
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Size1 ml
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CategoryPrimary Antibody
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Antibody SubtypePolyclonal Antibodies
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Area of researchMiscellaneous
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Type of ImmunogenBiotin antibodies were raised in rabbit using biotinylated KLH ..
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Raised inRabbit
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Cross ReactivityShould be determined by the end user
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Method of PurificationBiotin antibody was purified by ion exchange chromatography followed by dialysis.
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Form BufferIgG fraction Provided as a liquid in 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.2, and 0.01% NaN3 as a preservative.
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StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
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Shipping conditionsBlue Ice
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Tested forELISA; IHC; WB
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Usage RecommendationsELISA: 1:100,000, IHC: 1:2,000-1:10,000, WB: 1:2,000-1:10,000
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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French translationanticorps
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Short nameantibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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LabelBiotin
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Alternative nameRabbit polyclonal Biotin antibody
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data