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Category
Primary Antibody
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Antibody Subtype
Conjugated Polyclonal Antibodies
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Area of research
Miscellaneous
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Type of Immunogen
Biotin
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Raised in
Goat
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Conjugate Tag
FITC
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Grade Purity
Grater than 95% pure
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Method of Purification
Affinity purified using solid phase biotin
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Source
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration
1.0 mg/ml (lot specific)
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Form Buffer
Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
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Storage
Store at 2-8 deg C.
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Shipping conditions
Blue Ice
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Tested for
FC; IF; IHC
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Usage Recommendations
The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Biotinylated, Anti-FITC Antibody
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French translation
anticorps
