MHC Class I antibody (FITC)
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Catalog number
GEN530615
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Price
Please ask
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Size
200ul
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Also known as
MHC Class I (FITC)
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Other names
MHC class I; MHC class I; N/A; N/A; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
MHC Class I
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Gene name synonims
N/A
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Other gene names
N/A; gamr-UA-c24; N/A; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a
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Clone
3F10
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Host organism
Mouse (Mus musculus)
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Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Human; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
MHC Class I antibody (FITC) was purified by Protein A chromatography.
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Form Appearance
50mM Na3PO4, pH 7.5, with 100mM KCL, 150mM NaCl, 5% Glycerol, 0.2% BSA, and 0.04% NaN3.
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Concentration
0.25 mg/ml
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Storage and shipping
For optimal storage conditions, keep the antibody at +4°C. Temperature variations in the range 1°C to 9°C are tolerable.. Keep away from light.
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Tested applications
ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
MICD, MICF, MICG
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Short name
MHC Class I antibody (FITC)
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Anti-MHC Class I antibody (FITC)
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Alternative technique
antibodies, fluorescine
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Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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