Complement C5 Recombinant Protein

• Background :

  Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain) . Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

• NCBI Gene ID :

  103352022

• Accession Number :

  XP_008271599.1

• Tag :

  His Tag

• Type :

  Recombinant Proteins

• Source :

  HEK293 cells

• Purity :

  >90% as determined by SDS-PAGE.

• Buffer :

  PBS, pH7.4

• Shipping Conditions :

  Room Temp

• Storage Conditions :

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight :

  73.8 kDa (β chain) & 111.9 kDa (α chain)

• Applications Notes :

  This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. During cultivation, Complement C5 will be partially processed into α and β c

• NCBI Official Name :

  ComplementC5

• NCBI Organism :

  Oryctolagus cuniculus

• Background Reference 01 :

  DeMartino JA, et al. 1994. J Biol Chem. 269 (20) :14446-50.

• Background Reference 02 :

  Fredslund F, et al. 2008. Nat Immunol. 9 (7) :753-60.

• Background Reference 03 :

  Chen Z, et al. 1998. J Biol Chem. 273 (17) :10411-9.

• Other Product Names :

  Complement C5, C5, CPAMD4

• Tested Applications :

  WB

• Species :

  Rabbit

• Physical Properties :

  Lyophilized

• CAS Number :

  9000-83-3