Hydrogen Peroxide Colorimetric Detection Kit

Hydrogen Peroxide Colorimetric Detection Kit

• Description: Our Hydrogen Peroxide Colorimetric Detection Kit allows you to quantitatively measure hydrogen peroxide in a variety of samples. This kit is validated for use in fresh urine, buffers, and tissue culture media. This kit is species independent.
• Assay Principle: 1. Use plate layout template to aid in proper sample and standard identification., 2. Pipet 50 µL SSA-treated samples, standards, or control into plate wells., 3. Pipet 50 µL Sample Diluent into zero wells., 4. Add 25 µL Fluorescent GSH Detection Substrate to each well using a repeater pipet., 5. Gently tap plate sides to ensure adequate mixing of the reagents., 6. Incubate 15 minutes at room temperature., 7. Read the fluorescent emission at 510 nm with excitation at 370-410 nm. This data will be used to determine Free GSH concentration., 8. Add 25 µL Reaction Mixture to each well using a repeater pipet., 9. Gently tap plate sides to ensure adequate mixing of the reagents., 10. Incubate 15 minutes at room temperature., 11. Read the fluorescence with excitation at 370-410 nm and emission at 510 nm. This data will be used to determine Total GSH concentration.
• Shipping Conditions: Blue Ice
• Storage Conditions: 4°C