Nitric Oxide Colorimetric Detection Kit

Nitric Oxide Colorimetric Detection Kit

• Description: This kit offers two assays. The first quantitatively measures endogenous Nitrite. In the second, Nitrate is converted to Nitrite using Nitrate Reductase and Total Nitric Oxide is measured. Nitrate levels can be calculated by performing both assays.
• Assay Principle: 1. Prepare samples and controls., 2. Reconstitute ThioBright Green with 500 µL DMSO to produce a 200X stock concentrate., 3. Add ThioBright Green to each sample at 1:200. For example, add 5 µL to 995 µL cultured cells., 4. Incubate 15-30 minutes., 5. Optional: Spin and pellet cells, remove supernatants, and gently resuspend in Assay Buffer or other isotonic buffer., 6. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody., 7. Analyze with a flow cytometer. ThioBright Green excites at 490 nm and emits at 525 nm.
• Concentration: 5 mg/ml
• Shipping Conditions: Blue Ice
• Storage Conditions: 4°C