Hydrogen Peroxide Fluorescent Detection Kit

Hydrogen Peroxide Fluorescent Detection Kit

• Description: Our Hydrogen Peroxide Fluorescent Detection Kit is designed to quantitatively measure H2O2 in a variety of samples. This kit is validated for use in fresh urine, buffers, and tissue culture media samples. It is species independent.
• Assay Principle: 1. Prepare Assay Buffer; dilute Assay Buffer Concentrate 1:5 in diH2O., 2. Prepare samples; dilute > 1:10 in Assay Buffer., 3. Prepare standard curve and zero (blank) standard., 4. Add 50 µL blanks, standards, and samples to plate., 5. Add 25 µL Colorimetric H2O2 Detection Substrate to plate., 6. Prepare HRP, dilute Horseradish Peroxidase Concentrate 1:50 in Assay Buffer., 7. Add 25 µL HRP Preparation to plate., 8. Incubate 15 minutes at RT., 9. Read absorbance of the plate at 560 nm.
• Shipping Conditions: Blue Ice
• Storage Conditions: 4°C