Glutathione Colorimetric Detection Kit

Glutathione Colorimetric Detection Kit

• Description: Quantitatively measure reduced glutathione (GSH) and oxidized glutathione (GSSG) present in a variety of samples, including whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples. Analyze using a spectrophotometer.
• Assay Principle: 1. Prepare nitrate standard curve and blanks., 2. Add 50 µL Nitrate standards to plate., 3. Add 50 µL Nitric Oxide Assay Buffer, 1X to Zero wells., 4. Add 10 µL prepared NADH to each well., 5. Add 10 µL prepared Nitrate Reductase (NR) to each well., 6. Incubate 20 minutes at RT., 7. Add 25 µL Color Reagent A to each well., 8. Incubate 5 minutes at RT., 9. Read at 540-570 nm.
• Shipping Conditions: Blue Ice
• Storage Conditions: 4°C