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Hydrogen Peroxide Fluorescent Detection Kit

CAT: 0247-OKAU00031-2PLATESize: 2 PlatesDry Ice: NoHazardous: No
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CAT#:0247-OKAU00031-2PLATESize:2 Plates
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Sample H2O2 Conc. (uM) %CV 1 6.27 3.6 2 3.21 3.8 3 0.98 5.7 Sample H2O2 Conc. (uM) %CV Sample H2O2 Conc. (uM) %CV 1 6.27 3.6 1 6.27 3.6 2 3.21 3.8 2 3.21 3.8 3 0.98 5.7 3 0.98 5.7 Sample H2O2 Conc. (uM) %CV 1 5.86 4.3 2 3.00 7.0 3 0.88 12.1 Sample H2O2 Conc. (uM) %CV Sample H2O2 Conc. (uM) %CV 1 5.86 4.3 1 5.86 4.3 2 3.00 7.0 2 3.00 7.0 3 0.88 12.1 3 0.88 12.1
Gene Name
Hydrogen Peroxide
Type
Application Kit
Detection Method
Fluorometric
Assay Principle
The DetectX® Hydrogen Peroxide Fluorescent Detection Kit is designed to quantitatively measure H2O2 in a variety of samples. Please read the complete kit insert before performing this assay. A hydrogen peroxide standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Substrate and the reaction initiated by addition of horseradish peroxidase. The reaction is incubated at room temperature for 15 minutes. The HRP reacts with the substrate in the presence of hydrogen peroxide to convert the colorless substrate into a fluorescent product. The fluorescent product is read at 590 nm with excitation at 570 nm. Increasing levels of H2O2 cause a linear increase in fluorescent product.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Fresh Urine, Buffers and TCM
Sensitivity
Sensitivity was determined as 0.038 uM. This is equivalent to 1.9 pmol (64.6 pg) H2O2 per well.
Reconstitution
2°C to 8°C
Components
Component Quantity Black 96 well Half Area Plates 2 Plates Hydrogen Peroxide Standard 220 uL Assay Buffer Concentrate 25 mL Fluorescent Detection Reagent 5 mL Horseradish Peroxidase Concentrate 60 uL Component Quantity Component Quantity Black 96 well Half Area Plates 2 Plates Black 96 well Half Area Plates 2 Plates Hydrogen Peroxide Standard 220 uL Hydrogen Peroxide Standard 220 uL Assay Buffer Concentrate 25 mL Assay Buffer Concentrate 25 mL Fluorescent Detection Reagent 5 mL Fluorescent Detection Reagent 5 mL Horseradish Peroxidase Concentrate 60 uL Horseradish Peroxidase Concentrate 60 uL

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