IgM-Reducing Assay Diluent

CAT: 0246-623Size: 100 mLDry Ice: NoHazardous: No
CAT#:0246-623Size:100 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IgM-Reducing Assay Diluent is a specially formulated additive to address IgM-mediated conjugate bridging interference in problematic serum and plasma samples tested in antibody sandwich ELISA formats. IgM-Reducing Assay Diluent can be added to standard curve/calibrator and serum/plasma sample wells to help equalize matrix complexity disparity issues.
Assay Principle
1. Once the capture antigen has been coated onto the plate and the plate has been properly blocked with one of ICT’s stabilizing blocker products, pipette 50 – 100 µL of IgM-Reducing Assay Diluent into each of the wells of the ELISA plate using a multi-channel pipettor. The addition of the Assay Diluent is always done prior to adding the standard or test samples to each well., 2. The standard and test samples should be diluted to the proper well concentrations using one of ICT’s three different Sample Diluent products to help further equalize matrix complexity parameters between standard/calibrator wells and the serum/plasma containing sample wells., 3. Because IgM-Reducing Assay Diluent contains a more diverse composition of additives, it can have some negative impact on the antigen capture step of the ELISA. This leads to reduction in ELISA sensitivity which must be compensated for by using longer antigen capture incubation times or more sensitive HRP substrate components.
Concentration
1X
pH
pH: 7.4 at 1X
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
Expires 18 months from date of manufacture

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