General Assay Diluent

General Assay Diluent

• Description: A mammalian protein-based ELISA reagent to equalize sample and standard matrices for more accurate results. It reduces non-specific protein interaction with the plate, and reduces complement and thrombin interference in serum and plasma samples.
• Assay Principle: 1. Once the capture antigen has been coated onto the plate and the plate has been properly blocked with one of ICT’s stabilizing blocker products, pipette 50 – 100 µL of General Assay Diluent into each of the wells of the ELISA plate using a multi-channel pipettor. The addition of the Assay Diluent is always done prior to adding the standard or test samples to each well., 2. The standard and test samples should be diluted to the proper well concentrations using one of ICT’s three different Sample Diluent products to help further equalize matrix complexity parameters between standard/calibrator wells and the serum/plasma containing sample wells.
• Concentration: 1X
• pH: pH: 7.4 at 1X
• Shipping Conditions: Blue Ice
• Storage Conditions: 4°C
• Shelf Life: 24 months from date of manufacture