Assay Diluent Optimization Pack

Assay Diluent Optimization Pack

• Description: Assay Diluents are additive components used in an ELISA that equalize any differences between the sample matrices (serum, plasma, urine, cell culture fluid) and the calibrator diluent used to generate the standard curve of the ELISA. ICT’s Assay Diluent Optimization Pack, containing four 100 mL ELISA development buffers (General Assay Diluent, IgM-Reducing Assay Diluent, Neptune Assay Diluent, and Antigen-Down Assay Diluent), offers an economical method of addressing matrix effects and optimizing signal-to-noise ratio in ELISA development projects.
• Assay Principle: 1. To quickly determine which Sample Diluent formulation is best for the assay, all three Sample Diluents can be run in parallel on the same IgG or antigen-coated ELISA plate., 2. Determine the initial dynamic analyte range of the ELISA curves that were prepared in each of the three different Sample Diluent types., 3. Make test spikes into serum samples., 4. Read the spiked serum sample wells on each of the three SD-associated standard curves to see which Sample Diluent gives the best approximation of the spike concentration that was added to the serum samples., 5. In cases where a high level of matrix complexity interference is detected, inclusion of an additional Assay Diluent reagent may also be required to bring the calibrator well antibody-to-antigen binding kinetics closer to the sample well binding kinetics.
• Concentration: 5X
• pH: pH: 7.4 at 1X
• Shipping Conditions: Blue Ice
• Storage Conditions: 4°C
• Shelf Life: Expires 18 months - 24 monthsfrom date of manufacture