General Assay Diluent, 2X

General Assay Diluent, 2X

• Description: 2X General Assay Diluent is a mammalian protein-based ELISA additive used to equalize the sample and standard matrices for a more accurate result. Large differences in matrix complexity environment between standard curve and sample wells are the main cause for target analyte under-recovery problems in sandwich ELISA analysis. General Assay Diluent helps equalize matrix complexity disparity issues, reduces non-specific interactions between sample proteins and the plate, and reduces interference from complement and thrombin present in serum and plasma samples. Our 2X concentrated version is helpful for shipping purposes and produces less waste.
• Assay Principle: Milestones Common to All Sandwich ELISA Development Projects, 1. Acquisition of adequate quantities of quality antibody (IgG) and analyte components necessary to initiate project., 2. Preparation or acquisition of the sandwich IgG-HRP conjugates., 3. Demonstration of basic assay feasibility (dose response)., 4. Optimization of capture antibody coating., 5. Resolution of sample matrix interference issues., 6. Final optimization of sandwich IgG-HRP conjugate concentration, considering the inhibitory effects of the sample matrix., 7. Demonstration of ELISA performance capabilities via assessment of assay linear dynamic range, sensitivity, linearity, standard recovery efficiency, and precision., , For detailed protocols and information on each of these ELISA development milestones, please see the Antibody-Sandwich ELISA Development Kit Manual.
• pH: pH: 8.5-8.6
• Shipping Conditions: Blue Ice
• Storage Conditions: 4°C
• Shelf Life: 24 months from date of manufacture