Biotin-11-dUTP

• Description:

  Biotin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine.

• Product Name Alternative:

  Biotin-X- (5-aminoallyl) -dUTP

• UNSPSC:

  12352208

• UNSPSC Description:

  Nucleic acids

• HS Code:

  29349990

• eClass Number:

  34160990

• eClass Product Group:

  Nucleinsäure (nicht klassifiziert)

• Applications:

  Incorporation into DNA/cDNA by - PCR with Taq polymerase [1,2] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [3,4] & in-house data - Primer Extension with Klenow 3'-5' exo- [5] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[6] - Reverse Transcription with MMLV Reverse Transcriptase [7]

• Concentration:

  (1 mM)

• Purity:

  ≥ 95 % (HPLC)

• Form:

  Filtered solution (30 kDa) in 10 mM Tris-HCl

• pH:

  7.5 ±0.5

• Color:

  Colorless to slightly yellow

• Autoclavability:

  false

• Smiles:

  C1[C@H]2[C@@H] ([C@@H] (S1) CCCCC (=O) NCCCCCC (=O) NC/C=C/c1c (=O) [nH]c (=O) n ([C@H]3C[C@@H] ([C@H] (O3) COP (=O) (OP (=O) (OP (=O) (O) O) O) O) O) c1) NC (=O) N2

• Shipping Conditions:

  Shipped on gel packs

• Storage Conditions:

  Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Storage Temperature:

  Store at -20 °C

• Notes:

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life:

  12 months after date of delivery