DIG-11-dUTP

• Description:

  Digoxigenin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Digoxigenin-labeled DNA/cDNA probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine.

• UNSPSC:

  12352208

• UNSPSC Description:

  Nucleic acids

• HS Code:

  29349990

• eClass Number:

  34160990

• eClass Product Group:

  Nucleinsäure (nicht klassifiziert)

• Applications:

  Incorporation into DNA/cDNA by - PCR with Taq polymerase [1,2] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [3] and in-house data - Primer Extension with Klenow exo- [4] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [5] - Reverse Transcription with MMLV Reverse Transcriptase [6] Incorporation into RNA by - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [7]

• Concentration:

  (1 mM)

• Purity:

  ≥ 95 % (HPLC)

• Form:

  Filtered solution (30 kDa) in 10 mM Tris-HCl

• pH:

  7.5 ±0.5

• Color:

  Colorless to slightly yellow

• Autoclavability:

  false

• Smiles:

  C1[C@@H]2[C@] (CC[C@@H]1OCC (=O) NCCCCCC (=O) NC/C=C/c1c (=O) [nH]c (=O) n ([C@@H]3O[C@H] (COP (=O) (OP (=O) (OP (=O) (O) O) O) O) [C@@H] (O) C3) c1) ([C@@H]1[C@@H] (CC2) [C@@]2 ([C@] ([C@@H] (C1) O) ([C@H] (CC2) C1=CC (=O) OC1) C) O) C

• Shipping Conditions:

  Shipped on gel packs

• Storage Conditions:

  Store at -20 °C Short term exposure up to 1 week cumulative to ambient temperature possible.

• Storage Temperature:

  Store at -20 °C

• Notes:

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life:

  12 months after date of delivery