Biotin-16-dUTP

CAT: 0220-NU-803-BIO16-SSize: 200 µLDry Ice: NoHazardous: No
CAT#:0220-NU-803-BIO16-SSize:200 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Biotin-16-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of uridine.
Product Name Alternative
Biotin-16- (5-aminoallyl) -dUTP
Synonyms
Biotin-16-(5-aminoallyl)-dUTP
UNSPSC
12352208
UNSPSC Description
Nucleic acids
HS Code
29349990
eClass Number
34160990
eClass Product Group
Nucleinsäure (nicht klassifiziert)
Applications
Incorporation into DNA/cDNA by - PCR with Taq polymerase [1,2] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [3,4] & in-house data - Primer Extension with Klenow exo- [2,5] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [6,7] - Reverse Transcription with MMLV Reverse Transcriptase [8,9] Incorporation into RNA by - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [10]
Concentration
(1 mM)
Purity
≥ 98 % (HPLC)
Form
Filtered solution (30 kDa) in water
pH
7.5 ±0.5
Color
Colorless to slightly yellow
Autoclavability
—
Smiles
C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCC(=O)NC/C=C/c1c(=O)[nH]c(=O)n([C@H]3C[C@@H]([C@H](O3)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)NC(=O)N2
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C Short term exposure up to 1 week cumulative to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
Product Datasheet
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Product MSDS
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