DIG-11-dUTP

CAT: 0220-NU-803-DIGX-LSize: 5x 25 µLDry Ice: NoHazardous: No
CAT#:0220-NU-803-DIGX-LSize:5x 25 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Digoxigenin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Digoxigenin-labeled DNA/cDNA probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine.
UNSPSC
12352208
UNSPSC Description
Nucleic acids
HS Code
29349990
eClass Number
34160990
eClass Product Group
Nucleinsäure (nicht klassifiziert)
Applications
Incorporation into DNA/cDNA by - PCR with Taq polymerase [1,2] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [3] and in-house data - Primer Extension with Klenow exo- [4] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [5] - Reverse Transcription with MMLV Reverse Transcriptase [6] Incorporation into RNA by - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [7]
Concentration
(1 mM)
Purity
≥ 95 % (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl
pH
7.5 ±0.5
Color
Colorless to slightly yellow
Autoclavability
Smiles
C1[C@@H]2[C@](CC[C@@H]1OCC(=O)NCCCCCC(=O)NC/C=C/c1c(=O)[nH]c(=O)n([C@@H]3O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)[C@@H](O)C3)c1)([C@@H]1[C@@H](CC2)[C@@]2([C@]([C@@H](C1)O)([C@H](CC2)C1=CC(=O)OC1)C)O)C
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
Product Datasheet
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Product MSDS
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