Recombinant Mouse MCP-2 (CCL8)

• Description:

  Monocyte chemotactic protein (MCP-2) is a C-C chemokine. It shares over 60% amino acid identity with MCP-1 and MCP- 3 and has about 30% identity with other C-C chemokines MIP-1 alpha, RANTES, and MIP-1 beta. MCP-2 is chemotactic for and activates a wide variety of inflammatory cells. Also, its spectrum of action on leukocytes is similar to that of MCP-3. The leukocytes affected are monocytes, T lymphocytes, natural killer cells, basophils, mast cells, and eosinophils - differs from MCP-1 which is not active on eosinophils. MCP-2 has been proposed to interact with multiple C-C chemokine receptors, including those used by MCP-1 and MCP-3. However, since leukocytes express a multiplicity of chemokine receptors with promiscuous binding and functional properties, it is difficult to identify the receptors used by a given ligand on these cells.

• Gene Name:

  CCL8

• Gene Aliases:

  C-C motif chemokine 8, Monocyte chemotactic protein 2, MCP-2, Small-inducible cytokine A8, Mcp2, Scya8
  Recombinant Mouse Monocyte Chemoattractant Protein-2 (CCL8)

• Gene ID:

  20307

• Accession Number:

  Q9Z121

• Organism:

  Mouse

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  The ED(50) was determined by the dose-dependent chemo-attraction human PBMC using a range of 10-100 ng/mL.

• Molecular Weight:

  9

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Mouse MCP-2 was lyophilized from a 0.2 μm filtered 20 mM PB solution pH 7.5, 100 mM NaCl.

• CAS Number:

  9000-83-3