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Human CRYbB2 (Crystallin Beta B2) ELISA Kit

CAT: 0855-ELK5598-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5598-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRYbB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRYbB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CRYbB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRYbB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CCA2; CRYB2; CRYB2A; Beta-crystallin Bp
UniProt
P43320
Reactivity
Human
Field of Research
Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRYbB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRYbB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CRYbB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRYbB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.12 ng/mL

UniProtKB · P43320

Beta-crystallin B2

CRBB2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P43320
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CRYBB2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q9UCM8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Acetylation

Disease

CataractDisease variant

Molecular function

Eye lens protein

Domain

Repeat

Biological process

Sensory transductionVision

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