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Mouse CRYbB2 (Crystallin Beta B2) ELISA Kit

CAT: 0855-ELK7343-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7343-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CRYbB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CRYbB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CRYbB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CRYbB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CCA2; CRYB2; CRYB2A; Beta-crystallin Bp
UniProt
P62696
Reactivity
Mouse
Field of Research
Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CRYbB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CRYbB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CRYbB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CRYbB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.117 ng/mL

UniProtKB · P62696

Beta-crystallin B2

CRBB2_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P62696
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Crybb2
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
P19942, P26775, Q0VGT6
Protein keywords

Technical term

3D-structureReference proteome

PTM

Acetylation

Molecular function

Eye lens protein

Domain

Repeat

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