Simian BMG/b2-MG (Beta-2-Microglobulin) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin

• Gene Aliases:

  Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin

• UniProt:

  P63064

• Reactivity:

  Simian

• Field of Research:

  Infection immunity, Kidney biomarker

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  200 ng/mL

• Sample Type:

  Serum, plasma, urine and other biological fluids

• Detection Range:

  3.13 - 200 ng/mL

• Sensitivity:

  1.18 ng/mL

• Species:

  Simian

• CAS Number:

  7732-18-5