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Cattle CSN1 (Casein Alpha) ELISA Kit

CAT: 0855-ELK8008-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK8008-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CSN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CSN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CSN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CSN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CASa; CSN1; CSN1S1; Casein Alpha S1; Caseinate; Caseine/caséine/Kasein/caseína; Alpha-S1-casein; Casoxin-D
UniProt
P02662
Reactivity
Cattle
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CSN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CSN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CSN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CSN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Milk
Detection Range
0.16-10 ng/mL
Sensitivity
0.07 ng/mL

UniProtKB · P02662

Alpha-S1-casein

CASA1_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P02662
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CSN1S1
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A5YK80, A8WCP1, A8WCP2, A8WCP3, A8WCP4, A8WCP5, A8WCP6, A8WCP7, A8WCP8, A8WCP9, A8WCQ0, A8WCQ1, A8WCQ2, A8WCQ3, A8WCQ4, A8WCQ5, A8WCQ6, A8WCQ7, A8WCQ8, A8WCQ9, A8WCR0, A8WCR1, A8WCR2, A8WCR3, A8WCR4, A8WCR5, A8WCR6, A8WCR7, A8WCR8, A8WCR9, A8WCS0, A8WCS1, Q28048, Q28069, Q32LE8, Q7M2U6, Q9TRH5
Protein keywords

Disease

Allergen

Molecular function

AntioxidantMilk protein

Technical term

Direct protein sequencingReference proteome

PTM

Phosphoprotein

Domain

RepeatSignal

Cellular component

Secreted

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