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Cattle CSN2 (Casein Beta) ELISA Kit

CAT: 0855-ELK7989-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7989-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CSN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CSN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CSN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CSN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CASb; Beta-casein; CASB; CASB_HUMAN; Casein beta; CSN 2; Csn2; Casein beta S2
UniProt
P02666
Reactivity
Cattle
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CSN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CSN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CSN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CSN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 pg/mL
Sample Type
Milk
Detection Range
78.13-5000 pg/mL
Sensitivity
38 pg/mL

UniProtKB · P02666

Beta-casein

CASB_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P02666
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CSN2
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A1YQZ8, A6N8V0, Q2TA13, Q5EEQ6, Q5EEQ7, Q6UN63, Q9BDG5, Q9TSD5
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

AntioxidantHypotensive agentMetalloenzyme inhibitorMetalloprotease inhibitorMilk proteinProtease inhibitor

PTM

Phosphoprotein

Cellular component

Secreted

Domain

Signal

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